There are several approaches to analyzing qRT-PCR results. Below are some methods used by the Nicholas Lab and resources for performing the analysis.
* Delta Delta CT method – The delta-delta Ct method, also known as the 2–∆∆Ct method, is a simple formula used in order to calculate the relative fold gene expression of samples when performing real-time polymerase chain reaction. These calculations can be performed in an excel spreadsheet or with the aid of the CFX connect software at the data analysis station in lab.
*geNorm method – geNorm is a popular algorithm to determine the most stable reference (housekeeping) genes from a set of tested candidate reference genes in a given sample panel. From this, a gene expression normalization factor can be calculated for each sample based on the geometric mean of a user-defined number of reference genes. The geNorm method can take into account multiple housekeeping genes.